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The absorbance of the bacterial suspension was measured at 600 nm using a spectrophotometer at 0 and 2 h time points

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Then, 10 days after the application of the treatment, liquid nitrogen was used to freeze the fresh root and leaf samples, after which, the samples were stored at 80C until further analyses of the ROS, redox, and hormone levels

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[PMC free article: PMC3431581] [PubMed: 22962670] 663