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Single cells were harvested and washed with cold PBS, and fixed with fixation buffer (70% ethanol in PBS) overnight at 20 C

Conversely, the inhibition of the de novo synthesis of GSH dampens intracellular antioxidant defence mediated by this system and increases oxidative stress (Reference Lian, Gnanaprakasam and Wang28)

10.12688/f1000research.12111.1 162 SchirinziT.MartellaG.ImbrianiP.Di LazzaroG.FrancoD.ColonaV

Hypercholesterolemia impairs endothelium-dependent relaxations in common carotid arteries of apolipoprotein e-deficient mice

Furthermore, important questions remain, including the long-term durability of weight loss and its maintenance after treatment discontinuation, as well as its impact on major cardiovascular outcomes