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When the dominant bacterial species in the gut is altered, this results in instability of the gut microbiota and an immune reaction within the gut mucosa (82)

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First, mitochondrial dysfunction and metabolic abnormalities may act as potential initiating factors in PEM, manifesting as impaired ATP synthesis, overproduction of reactive oxygen species (ROS), and the accumulation of metabolic byproducts

All cell lines were maintained in a humidified atmosphere (containing 5% at 37 C) and in the following media supplemented with 10% fetal bovine serum (FBS): PA-TU- 8902 and Mia PaCa-2 in DMEM, BxPC-3 in RPMI

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