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Though possibly an appealing thought, steatosis should not be interpreted as being beneficial, since chronic hepatic steatosis is associated with several serious conditions, including dyslipidemia and hypertension, imposing considerable negative effects on the quality of life as well as increasing mortality in afflicted patients [76]

Prior to usage, the conditioned medium was supplemented with 50 ng/ml EGF (Peprotech, 315-09), 100 ng/ml noggin (Peprotech, 250-38), 1 g/ml R-Spondin-1 (Cat# GMP-CX83 Novoprotein, Shanghai, China), 10 M Jagged-1 peptide (Anaspec AS-61298), and 10% Afamin-Wnt-3A serum-free conditioned medium (MBL)