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The third group was incubated with N -Ethylmaleimide (NEM, a sulfite scavenger, 1 mM) for 30 min, then N -acetyl-L-cysteine (NAC, 1 mM) for 30 min to supply the endogenous generation of GSH, and DSNBD-GSH (10 M) for 30 min and imaged