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To remove unreacted FITC and peptides and exchange the peptide into the storage buffer (PBS), the reaction mixture was loaded onto an equilibrated Sephadex G10 column (1.5 150 cm) and eluted with PBS buffer, pH 7.5

Treatment duration at this dose typically ranges from 8-12 weeks, followed by an appropriate off-cycle period

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Conservative protocols involving cycling address this uncertainty by limiting continuous exposure

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