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Third, multicenter clinical sample repositories should be established, and retrospective cohort studies should examine associations between ferroptosis-related biomarkers (e.g., GPX4, ACSL4) and patient outcomes

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Grek CL, Zhang J, Manevich Y, Townsend DM, Tew KD (2013) Causes and consequences of cysteine S -glutathionylation

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In brief, a protein sample (50 mg) was dissolved in 15 mL of Tris-Gly buffer (8 mol/L urea, 50 L of Ellmans reagent)

Two endocannabinoids, anandamide and 2-arachidonoylglycerol, suppressed proliferation, induced apoptosis, deregulated the expression of cytoskeletal proteins (i.e., cytokeratins), upregulated lipid synthesis, and selectively activated the mitogen-activated protein kinase (MAPK) signaling pathway in human eccrine-sweat-gland-derived immortalized NCL-SG3 model cells