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2.2 Metabolite extraction of HCT116 cell lines Intracellular metabolites were extracted as previously described with some modifications (Dettmer et al., 2011)

coli ) strains DH5 and BL21(DE3) pLysS were grown in LuriaBertani (LB) broth at 37C in a shaking incubator or LB-agar at 37C

However, this acute oxidative stress serves as a hormetic stimulus that triggers adaptive responses, including upregulation of endogenous antioxidant systems including glutathione synthesis

10.1038/s41569-020-0352-5 196 SalabeiJ

Sah RP, Sharma A, Nagpal S, Patlolla SH, Sharma A, Kandlakunta H, et al

Seizure-induced reduction in glucose utilization promotes brain hypometabolism during epileptogenesis