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CONCLUSION: These results showed that longterm exposure of pancreatic islets to free fatty acid altered the dynamics of insulin secretion, probably through a glucosefatty acid cycle

Based on a randomized double blind, placebo controlled study of 135 adults over 12 weeks

Curr Opin Biotechnol 59:17

the five genes respectively involved in 6PGD , GCLC , GR , 5-OPase , and APX were upregulated from 1.49 to 10.28 (log2 FC)

Use of 100 ng of linear recombineering DNA fragments for allelic exchange produced 65 21 recombinant colonies, and correct recombination was confirmed in 62% 15% of colonies