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An amber stop codon was inserted at the C-terminus of D2 to allow for the expression of the soluble D1/D2-peptide fusion and a His-tag for purification

Low-level reactive oxygen species (ROS) are essential for intra- and intercellular signaling to maintain kidney homeostasis and function, including vascular reactivity, renal hemodynamics, glomerular filtration, tubular reabsorption and hormonal secretion, while excessive ROS are detrimental to renal cells by leading to oxidative stress [20]

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Patients with a mechanical aortic valve require a target INR of 2.0 to 3.0 to prevent catastrophic valve thrombosis or systemic embolization