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Supplementation of glucose-free media with L-cysteine instead of L-cystine led to increased intracellular levels of both L-cysteine and L-cystine, which may reflect either intracellular or extracellular oxidation of L-cysteine

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thus it seems highly likely that these processes occur in konzo, a motor neuron disease triggered in part by the consumption of toxic compounds that are well established to increase oxidative stress 43

Inhibition of serine biosynthesis through CRISPR- Cas9 -mediated knockout of PHGDH resulted in a ~250-fold reduction in the half-maximal inhibitory concentration (IC 50 ) for Rylaze, indicating heightened sensitivity to crisantaspase therapy