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Sections of 10 m were cut from the midbelly on a Leica CM1950 cryostat before immunostaining with type I, type IIa and type IIb myosin heavy chain isoforms (Developmental Studies Hybridoma Bank BA-F8, SC-71 and BF-F3) and laminin (Abcam 1575) (antibody details presented in Supplementary Table 3)

While these agents share overlapping indications with glycyrrhizin, their mechanisms of action are distinct yet potentially complementary, offering opportunities for synergistic combination strategies in the management of liver diseases (Li et al., 2021)

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Beyond effects on perforin, we noted that the availability of glutamine, the upstream substrate of ammonia metabolism which constitutes a fundamental metabolic prerequisite for NK-cell cytokine responsiveness (Terrn et al., 2019)