The final purified samples were desalted into phosphate buffer saline (PBS) for later tests and separated on a gel with a concentration of 12.5% SDS-PAGE
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The anterior chamber injection protocol was performed as described before [44]
It acts to block copper absorption in the intestinal tract and increase its excretion from the body
60mg Certification: WHO, GMP, ISO Dosage form: Powder for injection Package size: 1vial/box,
Disclosures: Virginia Clark: Nothing to Disclose, Charlie Strange: Takeda: Grant/Research Support, Grifols: Grant/Research Support, Beam: Consultant, GlaxoSmithKline: Consultant, Krystal: Grant/Research Support, Vertex: Grant/Research Support, AlphaNet: Executive role, Pavel Strnad: Arrowhead Pharmaceuticals: Grant/Research Support, CSL Behring: Grant/Research Support, CSL Behring: Speaking and Teaching, BioMarin Pharmaceutical: Consultant, GSK: Consultant, Intellia: Consultant, Takeda Pharmaceuticals: Speaking and Teaching, Novo Nordisk: Consultant, Swedish Orphan Biovitrum: Consultant, Swedish Orphan Biovitrum: Speaking and Teaching, IPSEN: Consultant, James Hamilton: Nothing to Disclose, Rong Zhou: Nothing to Disclose, Thomas Schluep: Nothing to Disclose, Anne Wyman: Nothing to Disclose, Susana Gonzalez: Takeda Pharmaceutical: Employee, Yanwei Zhang: Nothing to Disclose, Rohit Loomba: Nothing to Disclose 2524 PREVALENCE ESTIMATES OF GENOTYPES ASSOCIATED WITH ALPHA-1 ANTITRYPSIN DEFICIENCY: A RETROSPECTIVE COHORT STUDY IN THE USA Grahame Evans 1 Xinruo Zhang 2 Jennifer Below 1 Kari North 2 May Hagiwara 3 Chitra Karki 3 Tami Nussbaum 3 Suna Park 3 Kaili Ren 3 Virginia Clark 4 , 1 Vanderbilt University, 2 University of North Carolina Chapel Hill, 3 Takeda Development Center Americas, Inc., 4 University of Florida Background: Pathogenic SERPINA1 variants cause alpha-1 antitrypsin deficiency (AATD)
