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After liquid-nitrogen freeze-thaw, a Dounce homogenizer, 1mL (Active Motif) was used to crush the cardiac tissue, with the addition of 250 uL of lysis buffer (20mM HEPES, 0.01% SDS ( v/v ), pH 7.6, 1x PhosSTOP phosphatase inhibitor per 50mL and 1x Pierce Protease Inhibitor Mini Tablets per 10mL), before proceeding with probe sonication as described in Caenorhabditis elegans MS sample preparation

GPX4, a GSH-dependent enzyme, uses reducing equivalents from NADPH to detoxify lipid hydroperoxides

doi: 10.1158/1535-7163.MCT-08-0198 36 Holmquist-MengelbierLFredlundELfstedtTNogueraRNavarroSNilssonHet al

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The role of these metabolites in the development of hypertension has been reviewed elsewhere 147,148,149