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The cell lysates were centrifuged at 12,000 g for 10 minutes and the supernatant was mixed with the FLAG antibody overnight at 4C on a shaker, before incubation with prewashed protein A/G agarose beads at 25C for 4 hours

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Microbial bioactive metabolites represent a rich source of novel metabolites for the drug discovery and development

10.1016/S0168-1605(99)00022-7 47 VarelasV.TataridisP.LiouniM.NerantzisE