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In addition, lactate alone or combined with tumor-derived colony-stimulating factor-2 (CSF2) induces macrophage polarization towards tumor-associated macrophages M2-like macrophages, which are more supportive of tumor progression compared with immune surveillance polarization (M1) [128,131]

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To measure the CRT expressions, after treatment, the cells were washed with cold PBS, and further incubated with anti-CRT polyclonal antibody (diluted 1:100, anti-human antibody for G7, WL1, U87, and U251 cells and anti-mouse antibody for GL261 and G422 cells) at 4 C for 30 min, and stained with Alexa Fluor 647 labeled goat antirabbit IgG (H&L), a highly cross-adsorbed secondary antibody (1:200 dilution), for 30 min at room temperature

Study confirms that inhibition of mTORC1 suppresses SREBP1 transcription and promotes ferroptosis sensitivity of cells [96]