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Protocol for purifying biologically active microtubule severing protein UNC 45A from E.coli using GST affinity and spin columns: STAR Protocols Cytiva Glutathione Sepharose 4B Genesee Scientific Inhibitory probes for spatiotemporal analysis of Gs protein signaling Nature Chemical Biology G protein Gq subunits engage targets in the nucleus involved in chromatin remodeling and gene expression Journal of Biological Chemistry Frontiers GPI anchored glutathione S transferase as marker allows affinity sorting of transfection positive cells Munc13 4STX7 inhibitors impair endosomal TLR activation and systemic inflammation Nature Chemical Biology
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