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Cells (5 10 3 ) were plated in a 96-well plate in DMEM (A14430, Gibco, NY, USA) containing 5 mM glucose, 2 mM glutamine, and 1020% FBS

Besides regulating SNO-Parkin, SNO-Dj-1 itself also represents a potential PD therapeutic target since DJ-1 plays a role in activating the antioxidant response, cellular metabolism, cell survival, and redox homeostasis 160

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Several studies have indicated that NADH may react nonenzymatically with some xenobiotics and mediate their reduction 37,38

While honey is mostly sugar used for energy and propolis is a resin-like "bee glue" used for hive structural integrity, bee pollen is the nutritional powerhouse

Interventions included coordinating with neurologists for medication refills and consultations, adjusting appointment dates, and referring patients to hospitals if motor symptoms worsened