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Elimination is achieved by means of a mobile phase, typically combination of water and organic solvents (such as acetonitrile or methanol) are sprayed onto the column in the form of gradient, such as 95:5 aqueous: organic, for example and gradually increasing the organic phase until the elution buffer is 5:95 organic: aqueous)

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What you need Before starting, gather the following supplies

The 1-cm-long root segments were incubated in 10% KOH at 90C for 50 min, bleached by 10% H 2 O 2 for 15 min, acidified by 0.2 mol/L HCl for 10 min, and stained by 0.05% trypan blue in lactophenol for 30 s

Microscopic and Ultrastructural Changes of the Intestine in Abdominal Compartment Syndrome