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The output was collected and then concentrated and washed with DI water using 3 kDa molecular weight cut-off (MWCO) filtration tubes (Sartorius Stedim Biotech, Germany)

Does it improve recovery

Protein provides the amino acids glycine and glutamic acid, but cysteinethe third amino acid neededis often harder to get in sufficient amounts

Chemokines: CCL2 and CXCL12 Chemokines play a crucial role in immune cell trafficking and have been implicated in depression

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ALCAR and KET also activated brainstem nuclei (locus coeruleus, diagonal band, and raphe nuclei) which send aminergic projections to cortical areas and spinal dorsal horns that are involved in pain and mood control (Freo et al., 2010