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At each time point, 1/10 volume of culture medium per well CCK-8 solution reagent was added, incubated at 37C for 3 h, and the absorbance was measured at 450 nm using a microplate reader

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Notably, nuciferine, the primary bioactive compound isolated from lotus leaf, can prevent iron accumulation and lipid peroxidation in folic acid-induced AKI by enhancing intracellular GSH and GPX4 levels, ultimately inhibiting ferroptosis (166)

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NADPH plays important roles in anabolic processes including fatty acid, amino acid and nucleotide synthesis (98)

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