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Expression of the NRF2-target genes HMOX1 and NQO1 was reduced in ERK5-silenced MMH

These proteinenzyme samples were incubated at room temperature (~20 C) for 4590 min with rotation prior to supernatant elution by centrifugation in microflow columns (Thermo Fisher Scientific, Waltham, MA)

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Endogenous IGF-1 is suppressed during use (Tomas et al., 1997) and recovers across the off-period

Additionally, another NADPH-generating enzyme IDH1 was found to be crucial for the anchorage-independent growth, a property shared by many malignant cells