Behavioral assessments (open-field, self-grooming, social interactions) were followed by biochemical and molecular analyses in the cerebellum, where oxidative stress markers (MDA, GSH, SOD, CAT, and GPx) were quantified spectrophotometrically, and Nrf2, Bax, Bcl-2, and Caspase-3 mRNA levels were measured by q RT-PCR
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Crocin, crocetin, picrocrocin, and safranal are the principal active compounds in saffron which exhibit a wide spectrum of biological activities including analgesia, antioxidant activity, cardiovascular protection, and inhibition of cancer, diabetes, inflammation, and depression, among others [518, 519]
Disponible sur: Acknowledgements The authors thank Professor Aurlien Thomas, PhD (Unit de Toxicologie et Chimie Forensiques, Lausanne, Switzerland) for assisting with iron quantification in ocular fluids and Elise Orhan, PhD, Pauline Gondouin, and Marie Piazza for assisting with the development and validation of the immunoassay for the quantification of human transferrin in aqueous humor samples
AUTHORS CONTRIBUTIONS All authors contributed greatly to the success of this work CONFLICT OF INTEREST No conflict of interest to declare in this manuscript ACKNOWLEDGEMENT This case was carried out without direct funding, and the authors would like to thank the Head and the entire staffs of the Department of Human Physiology, College of Medical Sciences, Ahmadu Bello University, Zaria, for their support and provision of necessary facilities
viz., Hepatocellular Carcinoma development, which was further validated by IPA match analysis