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Because the peptide is naturally present in the body rather than purely synthetic, researchers use its measurable decline to compare younger and older tissue environments under controlled conditions similar in principle to Pickarts original liver-cell experiments

The slides were mounted with a solution containing DAPI (Molecular Probes) and imaged using a Leica TCS SP8 laser scanning confocal microscope (Leica Microsystems CMS GmbH, Wetzlar, Germany)

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