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a screening criterion is the selection for a variant of an antibody comprising a human IgG Fc region, which variant does not activate complement and comprises an amino acid substitution at amino acid position 322 or amino acid position 329, or both amino acid positions of the human IgG Fc region, where the numbering of the residues in the IgG Fc region is that of the EU index as in Kabat, and wherein the variant retains the ability to bind antigen

The polynucleotide to be shuffled can be produced as random or non-random polynucleotides, at the discretion of the practitioner

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Single-voxel 1 H MR spectra were acquired from a volume of interest (VOI = 20 20 25 mm 3 ) in the medial prefrontal cortex using a short-TE spin-echo full-intensity acquired localized single voxel spectroscopy technique (SPECIAL) with the following scan parameters: TE/TR = 6/4000 ms, acquisition bandwidth = 2 kHz, number of averages = 148, vector size = 2048

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On the connected Orbitrap Exploris 480 (Thermo Fisher, Waltham, MA), the MS and MS/MS automatic gain control targets were set to 300% and 100%, respectively, or maximum ion injection times of 50 ms (MS) and 30 ms (MS/MS) were used