Los polipptidos de FX modificados o el polipptido de FX modificado aislado proporcionado en el presente documento puede contener solo dos, tres, cuatro, cinco, seis, siete, ocho, nueve, diez, once, doce, trece, catorce, quince, diecisis, diecisiete, dieciocho, diecinueve o veinte sustituciones de aminocidos en comparacin con el polipptido sin modificar de la misma forma, por ejemplo, el polipptido sin modificar que tiene la secuencia de aminocidos que se muestra en la SEQ ID NO:134 o el zimgeno, la forma activa o catalticamente activa del mismo.In any of the examples of the modified FX polypeptides or the isolated FX polypeptides provided herein or described above, the polypeptide may contain one (s) substitution (s) of additional amino acid (s) in the light chain or in the protease domain of the heavy chain
Ironsulfur cluster biogenesis and human disease
Thereafter, 5 L of 3% H 2 O 2 and 914 L of dH 2 O (final concentration 4.8 mM) were mixed, and 90 L of this 50 M substrate was added to these wells to initiate the CAT reaction and incubated for 30 minutes at room temperature
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Pylori, as the bacteria in the H
