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Bound proteins were eluted by boiling in 2 SDS loading buffer

A smaller increase in 2,3-DPG was observed in aplastic anaemia as compared with other forms of anaemia (134)

Then, cDNA was synthesized using the RevertAid cDNA synthesis kit (Thermo Scientific, Rockford, IL, USA) according to the manufacturers instructions

BPC-157 BPC-157, also known as Body Protection Compound-157, is a peptide that is naturally found in human gastric fluids

7 Conclusion Supraspinatus muscle atrophy secondary to rotator cuff injury represents a progressive and frequently irreversible degenerative process that profoundly compromises shoulder function and limits postoperative recovery

Hum Mutat 22, 103 (2003)