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Next, the At LDOX protein was removed using an Amicon Ultra-4 device (10 kDa cut-off) (Merck Millipore) and the eluent was diluted with 1.375 vol of the same reaction buffer containing 82% H 2 18 O and incubated at 30 C, 1,200 rpm using a 3 mm shaking diameter to achieve 18 O equilibration

Yang Q, Cong L, Wang Y, Luo X, Li H, Wang H, et al

Preprocessing The gene expression microarray dataset was preprocessed using the R programming language, including gene name annotation, handling of missing values, and dealing with duplicate entries

The good news is, early studies and clinical experience suggest it can be a safe and effective tool when used correctly and under professional guidance

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doi: 10.3390/pr10040702 40 FathiFEbrahimiSNMatosLCOliveiraMBPPAlvesRC