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For long-term storage of reconstituted material, aliquot into single-use volumes and freeze at 80 C to avoid repeated freeze-thaw cycles, which are the single largest source of variability in peptide assays

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The journal of physical chemistry B 113 (2009) 1505715066

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With age, nighttime melatonin often drops, disrupting the natural sleep-wake cycle and indicating a less active pineal gland

Molecular Metabolism, 30, 72-130