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To mechanize the complex molecular assembly and preparation of the input files for the MD simulation under periodic boundary conditions, we used a Multicomponent Assembler in CHARMM-GUI 122

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Higher sGPVI levels (Q4 vs

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Almost 100% of Rg GUS, Lr GUS, and Fp GUS eluted in single peaks with weight-average molar masses of 291.5 kDa, 284.2 kDa, and 293.0 kDa, respectively, indicating that they form stable tetramers in solution

A 0.5 ml portion of each assay preparation was added to a well in a 24-well plate, and they were incubated at 37 C in 5% CO 2 for 1 h