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For histological examination, H&E staining was carried out

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Biochem Soc Symp 61:131 Davies MJ (2016) Protein oxidation and peroxidation

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Cells were fixed immediately in 4% paraformaldehyde for 20 min at room temperature, washed thrice with PBS, incubated for 1 h in blocking buffer (3% BSA, 0.5% saponin in PBS) and probed overnight at 4 C with anti-cytochrome c antibody (BD Pharmigen 556432, 1:200 in blocking buffer)