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Its significant inhibitory effect on immune function makes it unsuitable for treating long-term chronic diseases, including cardiac hypertrophy and heart failure [11]

10.1080/15548627.2016.1187366 Autophagy 40 IkedaM

coli (IC 50 ~ 27.5 g mL 1 ) and Pseudomonas cichorii (IC 50 ~ 13.8 g mL 1 ) ( Spodoptera litura larvae and Henosepilachna vigintioctopunctata (Murata et al., 2021)

10.1074/jbc.M310704200 11 FerreiraA.BallaJ.JeneyV.BallaG.SoaresM

Proteins were then precipitated by addition of 500 l methanol (pre-cooled at 80 C), 400 l LCMS-grade water, and 100 l chloroform, and the mixture was vortexed and centrifuged (10,000 g, 10 min, 4C) to afford a protein disc at the interface of CHCl3 and aqueous layers