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The percentage of time spent with the novel object was calculated by dividing the time the mouse spent exploring the novel object (O1) by the sum of the time spent exploring the novel object (O1) and the familiar object (O2) and then multiplying the result by 100 [110, 111]

Preventive measures should involve lifestyle modifications, such as maintaining a healthy diet, engaging in regular physical activity, and quitting smoking, as well as pharmacological interventions to control blood pressure, blood glucose, and lipid levels (Pasquel et al., 2018

first proposed the concept of oxidative stress, either caused by an increase in free radical production or a reduction in the scavenging capacity of the body, leading to disorders in the oxidation and antioxidant systems, resulting in oxidative damage by free radical accumulation (Sohal and Allen, 1990)

The supernatant was then analyzed by HPLC with a Rainin Econosphere ODS C18 column and an acetonitrile/water mobile phase containing 0.1% trifluoroacetic acid