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At the end of the experiment (48 hours), 100 l were transferred to a cytometer tube, 0.5 l of 25 M Calcein-AM were added and, after 15 min of incubation at room temperature, 5 l of fluorescently labeled beads plus 195 l of PBS 1X were added and cells were analyzed by flow cytometry

An interesting HPLC approach, suitable for searching natural antioxidants in plant extract of Flos Lonicerae Japonicae, was developed by Tang et al

Finally, once externalized, succinate can bind to the G protein-coupled succinate receptor 1 (SUCNR1) that is expressed on the surface of activated M1 polarized macrophages [111, 112]