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We did not receive any public comment on our proposed burden assumptions and are finalizing as proposed

Tocopherols in the leaves were quantified using high-performance liquid chromatography (HPLC) with Dimethyl tocol serving as an internal standard, as outlined by AbdElgawad et al

I did 3-4 rounds and used lipo for the last one

GSH and GSSG assay Reduced (GSH) and oxidized (GSSG) glutathione measurements were performed using a GSH/GSSG-Glo Assay Kit (Promega)

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However, when environmental conditions become stressful, such as high UV exposure, nutrient depletion, or changes in salinity, the algae shift into a survival mode