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Description

Chromatin was extracted by the addition of 1 ml of NUN2 buffer containing 20 mM HEPES (pH = 7.6), 7.5 mM MgCl 2 , 0.2 mM EGTA, 300 mM NaCl, 1 M urea and 1 % NP-40, 1x protease and 1x phosphatase inhibitors and rotated at 4 C for 10 min

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10.1007/s12015-022-10398-w 25 CicconeL.PiragineE.BrogiS.CamodecaC.FucciR.CalderoneV.et al (2022)

Pharmacokinetics of aspirin and salicylate in relation to inhibition of arachidonate cyclooxygenase and anti-inflammatory activity

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Our in vitro studies and HSAB analyses indicated that, as a soft nucleophile, 2-ACP was able to effectively scavenge NAPQI, the reactive soft electrophilic APAP metabolite