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The intranasal delivery of Apelin-13 provides a noninvasive method for directly administering the peptide therapy to the brain and bypassing the BBB [155]

Mo Med 115:219 Watari J, Chen N, Amenta PS, Fukui H, Oshima T, Tomita T et al (2014) Helicobacter pylori associated chronic gastritis, clinical syndromes, precancerous lesions, and pathogenesis of gastric cancer development

Recombinant protein purification and in vitro binding assays GST, GST-mSIN, GST-AKT (K179D), GST-PTPN22, His-tagged PTPN22, MBP or MBP-mSIN, MBP-PTPN22 full-length proteins were expressed in Escherichia coli BL21 (DE3) cells, and the proteins were affinity purified using either glutathione sepharose TM 4B beads (GE Healthcare, GE17-0756-01) [for GST-tagged proteins] or dextran sepharose TM beads (GE Healthcare, GE28-9355-97) [for MBP-tagged proteins] or Nickel NTA agarose beads (Qiagen, 30210) for 2 h at 4 C

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