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It is also contemplated that a number of cycles of nucleic acid shuffling may be conducted with a mixture of wild-type polynucleotides and a sub-population of nucleic acid from the first or subsequent rounds of nucleic acid shuffling in order to remove any silent mutations from the sub-population

And then we got even broader to several SNPs

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If you start with a low-purity product, you're already fighting an uphill battle

Similar binding patterns were observed for ondansetron, with a hydrogen bond with ASN193 (2.2 ), a salt bridge with GLU200 (4.8 ), pi -cation interaction with LYS292 (4.2 ), and a pi -pi interaction with PHE280 (3.8 ) as indicated in Figure 2