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Cell proliferation assay The viability of GC-1 spg, TM3 Leydig, and TM4 Sertoli cells was measured using an MTT assay EZ-Cytox kit (Daeil Lab Services Co., Seoul, Korea) according to the manufacturers instructions Each cell was seeded in 24-well plates at a density of 0.5 10 5 cells/well and incubated at 37 C for 12 h

Description of Method and Comparison of Ascorbic Acid Levels in Whole Blood, Plasma, Red Cells, and White Layer

10.1254/jphs.95.19 [DOI] [PubMed] [Google Scholar] Luetic K., Sucic M., Vlainic J., Halle Z

Decreased arginine levels or excessive NO production suppress T cell proliferation and promote Treg differentiation

However, therapeutic application of SIRT1 activators is not without risk

Diabetologia 58 , 3136 (2015)