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Curcumin inhibits angiogenesis and improves defective hematopoiesis induced by tumor-derived VEGF in tumor model through modulating VEGF-VEGFR2 signaling pathway

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doi: 10.1038/s41467-023-37849-3 173 WuY.JhaR.LiA.LiuH.ZhangZ.ZhangC.et al

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AP: Methodology, Resources, Writing original draft, Writing review & editing, Investigation

After observing leakiness of the GFP from the ER to the Golgi at the steady state, due to the presence of free biotin coming from DMEM/F-12 and B27-Supplement, we sequestered it by supplying 0.1 mg/ml of NeutrAvidin Protein (Thermo Scientific, #31000) to the WiBi media for 2-3 days before performing live imaging