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RNA (0.5 g) was reverse-transcribed in 20 L of 1 buffer and 1.25 M of random primers using SuperScript III Reverse Transcriptase (Thermo Fisher Scientific, Massachusetts, USA, 18080-093#) for 10 min at 30 C, 30 min at 42 C, and 5 min at 99 C, according to the manufacturers instructions

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Lee et al., 1989

Transcription factors such as AP-1, SOX2, FRA-2, and JUND were implicated in the regulation of cell differentiation, with AP-1 binding sites overlapping those of FRA-2 and JUND, suggesting their collaborative role in gene regulation