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The slides were treated with a lysis solution (2.5 M NaCl, 100 mM EDTA, 100 mM TRIS, 1% Triton X-100, 10% DMSO, pH ~ 10.2) for 24 h at 4C

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Its four-amino-acid simplicity belies a potentially profound mechanism if its telomerase activation effects translate robustly to human biology, it would represent one of the most direct anti-aging interventions studied to date

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One-half was dephosphorylated using rAPid alkaline phosphatase (Sigma-Aldrich