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Two separate rounds of filament picking on the entire dataset resulted in 2,374,969 and 2,865,445 particles, which were eventually merged into a pool of 492,224 particles after many rounds of 2D classification and removal of duplicate particles

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Before the procedure, you must follow the given instructions to get better outcomes: Hydrate: Drink two large glasses of water before having the appointment. Balance meal: Eat a balanced diet, with carbohydrates and protein before you go to prevent blood sugar drops during the method