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Colonies with Hif1a KO were selected by flow cytometry single cell sorting and expression validated by immunoblotting

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At 60 h-post injection, the mice were sacrificed and the biodistribution determined upon fluorescence imaging of the respective organs

While Tax4Fun offers an insight into potential metabolic capabilities of a system, follow-on functional studies would be needed, such as expression studies and metabolomics, to fully understand the function of microbial communities in brine

Platelets: no longer bystanders in liver disease

94 , e0001620 (2020)