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The resulting precipitate was separated by centrifugation at 7.245 g for 10 min, dialyzed against dH 2 O, and dried at 60 C until a constant weight was achieved

In the last step, a colorimetric substrate is added, which is digested by the enzyme

On the other hand, beyond its acute insulinotropic effects, GLP-1 promotes -cell proliferation and inhibits apoptosis (Liu Y

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These mechanisms collectively contribute to its efficacy against oral pathogens such as Streptococcus mutans, Candida albicans, and Gram-negative bacteria, reinforcing its role as a versatile antimicrobial adjunct in oral health applications