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SYTOX cell death assay Macrophages were seeded in a 96-well plate (2.5 10 4 cells per well in 100 L) and incubated at 37 C and 5% CO 2 overnight to allow adherence

References Kostrzbska A, Junka A, Broyna M, Musia W

For the human orthotopic injection model, TS543 cells (10 5 cells in 2 L, passage 8) were injected in 8-week-old female nude mice (CrTac:NCr- Foxn1 nu , Taconic) using the same co-ordinates as above

fragilis can release a unique pro-inflammatory LPS-subtype and fragilysin, leading to an impaired intestinal paracellular and transcellular epithelial barrier, thereby allowing an already leaky barrier a gateway for the enhanced entry of microbiome-derived neuro- and entero-toxins into the circulation (Lukiw et al., 2021)

Kalluri JR et al

[Google Scholar] 72.Zargar B and Hatamie A, Anal