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In the caput epididymis, the function of H 2 S is probably in reducing sperm motility by increasing the extracellular concentration of K + ions, keeping them in a quiescent state before ejaculation ( H 2 S production has also been described in vas deferens ( 2 S-producing enzymes have been detected in the prostate, with the highest abundance of CTH, which is likely the main H 2 S producer in this tissue (113, 114)

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As outlined above, studies on non-glutathione disulfide substrates unfortunately regularly lack more detailed kinetic analyses, first, to confirm the efficient turnover of the substrate candidates and, second, to validate the dithiol mechanism