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This analysis included global data normalization by equalizing the total peak/reporter areas across all LC-MS runs, data imputation using the knn algorithm, summation of peak areas per protein and LCMS/MS run, followed by calculation of peptide abundance ratios and testing for differential abundance using empirical Bayes moderated t-statistics (as implemented in the R/Bioconductor limma package)

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Intriguingly, new research indicates that colonic INSL5 expression is lowered in GF mice, whereas microbial colonization or supplementing with an HFD increases INSL5 levels, and that INSL5-KO animals have increased insulin tolerance and reduced hepatic glucose production 58

10.2217/pgs.15.183 [DOI] [PubMed] [Google Scholar] Angus WG, Mousa MA, Vargas VM, Quensen JF, Boyd SA, Contreras ML, 1997

HIV-1 proteins, including Tat, Nef, Vpr, protease, and gp120, modulate ROS-generating systems and induce oxidative stress, thereby promoting the expression of viral genes and proinflammatory lymphokines 114,115,116