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Sies and Cadenas (1985) introduced the term from the book

The hypothalamus detects rising blood sodium and turns on persistent thirst

It also must not be used for fluid replacement or volume expansion, and not for epidural or spinal anesthesia

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There are other, perhaps more common, genetic variants associated with the risk of developing ADincluding the late onset form of the disease (LOAD)such as ApoE4 [521, 522] or those identified more recently (e.g., mutations in CD36 [523], see [524] for review)

After 60 min incubation the samples were washed 2x with 1 ml of binding buffer (125 mM Tris-HCl (pH = 8.0), 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.1% Tween 20), mixed with 1 ug of SNIP1 peptide in 300 ul of binding buffer and rotated for 60 min at 4 C