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The results showed that the combined use of these vitamins have a positive effect on the reduction of Hcy concentration, thus, the daily doses of 5 mg folic acid, 1 mg B12, and 25 mg B6 for 18 months can reduce homocysteine levels by 26 % (from 9.2 to 6.78 mol/L) [189]

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The shRNA sequence (TRCN0000322995) targeting ATG10 was designed according to MISSION shRNA Plasmid DNA (MERCK) and synthesized and joined the FastDigest BshTI/FastDigest EcoRI linearized (Thermo Fisher) pLKO.1-GST DAF vector with T4 DNA ligase (EL0011, Thermo Fisher)